COMPARATIVE ANALYSIS OF BACTERIOCINS PRODUCED BY PSEUDOMONAS AERUGINOSA AND BACILLUS BREVIS

摘要

An attempt is made to comparatively analyse the effectiveness of bacteriocins produced by the indigenous soil bacteria namely Pseudomanas aeruginosa and Bacillus brevis against selective pathogens such as Staphylococcus aureus, Streptococcus pyogens,Enterococcus faecalis and Corynebacterium diptheriae by measuring the zone of inhibition. The bacteriocins are bacillocin of B.brevis and pyocin of P.aeruginosa. Both the bacteriocins effectively acted against the pathogens and comparatively pyocin of P.aeruginosa gave better results than bacillocin of B.brevis. But the effectiveness of bacillocin was particularly greater against Corynebacterium diptheriae (20mm) than the effectiveness of pyocin (10mm). Also the determination of bacteriocin production in growth curve revealed that the production of bacteriocins started during early exponential growth phase and continued till late stationary phase. Maximum production of bacillocin and pyocin was found at 11 hours of incubation.