ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF HIGH SALT TOLERANT BACTERIA WITH CMCase ABILITY

摘要

A Single bacterial colony was isolated by plating the aseptically separated crushed-cuticle sample of Penaeus monodon on an agar solidified LB medium, supplemented with 10 g/100 mL salt concentration. The bacterium was found to be gram positive, non-motile, aerobic and lactose non-fermenting through morphological and biochemical tests. The optimum culture condition of the isolate was at pH 7.5 with temperature of 30° C. The bacteria showed the ability of utilizing arabinose, sucrose, maltose, fructose as carbon sources and viable cell count was 489xl07CFU/mL. Isolated bacterium showed citrate metabolism ability, the ability of breaking down the amino acid tryptophan into indole and no catalase activity. The bacterium was resistant to amoxicillin, penicillin and vancomycin and the MIC value against penicillin was lOOmg/mL. The bacterial colony was also specially characterized for CMCase production activity. The molecular weight of isolated bacterial genomic DNA was above 10 kb with not much smearing. High salt tolerance ability up to 12g/100mL medium was observed while culturing on different salt concentrations. Purpose of the study was to screen out a potential source of salt tolerant gene with CMCase activity.