IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PHYTOPLASMA ASSOCIATED WITH PEACH DISEASE IN WESTERN UTTAR PRADESH

摘要

Recently, peach trees were showing symptoms of disease caused by phytoplasma, like leaf reddening, yellowing, leaf rolling symptoms were observed in peach growing areas in western regions of Uttar Pradesh. In nature phytoplasma are transmitted by leafhopper that may have occasional or permanent trophic relationship with their host. Plant pathogenic phytoplasma are wall less, unculturable bacteria 6f class mollicates with a small genome size, which ranges from 530 to 1350 kilobases. Total genomic DNAwas extracted from Different parts of peach tree by CTAB methods and with DNA isolation kit Quiagen (Germany). The Polymerase chain reactions (PCR) were performed using Phytoplasma universal primer pairs. No PCR amplification was observed in the healthyplants. Amplification of phytoplasmal 16S rDNA gene, 16S-23S intergenic spacer region and 5' region of the 23S rRNA gene sequence in PCRs containing phytoplasma universal primer pairs P1/P7, R16F2n/R2 and PI/ Tint and template DNA extracted from diseasedpeach plant confirmed that the plant were infected by phytoplasma. The Phylogenetic relation to other Phytoplasma was evaluated through iphyclassifier.