A rapid HPLC method is described for benziodarone assay in human plasma using a LiChrosorb RP-18 column and UV detection at 240 nm. Protein precipitation is followed by extraction of benziodarone and khellin (internal standard). Extraction of the active substances with diethyl ether at pH ca. 4.8 ensures fairly good recovery (91.04%, mean). Detection limit for this method of determination is 20 ng using a 1 ml sample. The method is specific and can also be used for the determination of benziodarone in Pharmaceuticals (tablets).
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