TetR-Type Regulator Lp_2642 Positively Regulates Plantaricin EF Production Based on Genome-Wide Transcriptome Sequencing of Lactiplantibacillus plantarum 163 br

摘要

Whole-genome and transcriptome sequences ofLactiplantibacillus plantarum163 are provided. There was onecircular chromosome and four circular plasmids, with sizes of 3,131,367; 56,674; 49,140; 43,628; and 36,387 bp, respectively, inL.plantarum163. The regulator Lp_2642 was selected from the genome data, the overexpression of which increased the transcriptionallevels of related genes in plantaricin EF biosynthesis and enhanced plantaricin EF production. Its production was 17.30 mg/L in 163(Lp_2642), which was 1.29-fold higher than that of the original strain. The regulation mechanism demonstrated that Lp_2642 canbind to three sites ofplnApromoter, which enhances its transcription and expression, thereby increasing plantaricin EF production.Amino acids Asn-100, Asn-64, and Thr-69 may play a key role in the binding of Lp_2642. These results provide a novel strategy formass production of plantaricin EF, which facilitates its large-scale production and application in the agriculture and food industries asa preservative.