Complement‐dependent cytolysis of human B lymphocytes with anti‐light chain antisera

摘要

AbstractUsing anti‐light chain antiserum and rabbit complement, 12 % of human peripheral blood lymphocytes and 42 % of tonsillar lymphocytes were specifically lysed. From control experiments it could be assumed that these cells are B lymphocytes. Under comparable conditions, cytolysis of tonsillar lymphocytes on a preparative scale was achieved. After removal of the lysed cells by Ficoll‐Isopaque centrifugation, the T/B cell compositions of the remaining living cell population and the untreated cell suspension were compared using the immunofluorescence technique for demonstration of surface immunoglobulin‐bearing cells and the rosette tests for sheep erythrocyte‐binding and complement‐receptor leukocytes. A maximal B cell depletion of 97 % and a T cell enrichment up to 139 % could be obtained. The applicability of the described method for studies on lymphocyte functions is