Usefulness of several commercial enzymehyphen;linked immunoassays for detection ofHelicobacter pyloriinfection in clinical medicine

摘要

Objective:To study six commercial enzyme-linked immunosorbent assays (ELISA) for detection ofHelicobacter pyloriimmunoglobulin (lg)G antibodies.Design:A comparison of different ELISAs in patients with knownH. pyloristatus.Patients:Patients who underwent endoscopy during which biopsy specimens were taken for detection ofH. pylorivia standard histologic and microbiologic techniques.Results:Positive and negative predictive values for the different ELISAs are comparable, but major inter- and intra-assay variability was present.Conclusion:Commercial assays should be tested with a local reference population in order to obtain the optimal cut-off value via a receiver operating characteristics curve. The use of the cut-off values provided by the manufacturers introduces difficulties with interpretation and is not valid.