Micropipette is a quite simple, but an essential instrument used for the volume measurements and finds the applications in the field of chemistry, pharmacy, health care, biology, pathology, research and development, etc. The calibration laboratories must ensure that the results obtained using these instruments are accurate, and therefore, the calibration is an inevitable process. This report is on the micropipette calibration techniques with different methods considering various influencing factors those affect the measurement results. The measurements were done in controlled environmental conditions and taking all the necessary precautions. This report also presents a detailed comparison between the three distinct test methods used, i.e. "conventional method", "with evaporation loss correction" method and "without evaporation loss correction" method. This report summarizes how the change of techniques applied can affect the final volume of the micropipette and the associated uncertainty in the measurement. This study will contribute to the improvement of the whole process of micropipette calibration for performing the calibration in volume. Further such studies can also be conducted for glassware calibration to improve the overall upgradation of volume calibration.
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