Oxime derivatives of a number of steroidal 4-en-3-one 17-alcohols and 17-esters have been separated into syn and anti isomers by HPLC under normal or reverse-phase conditions. The elution order of the isomeric pairs was found to vary with the nature of the steroid and the type of stationary phase: a peak ratio method at two wavelengths provides a very convenient method for unambiguously identifying the geometries of the isomeric pairs.
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