Improved Technique for Detecting Australia Antigen by Immunoelectroosmophoresis1

摘要

Abstract.Comparison between the sensitivity in detecting Australia antigen of the Ouchterlony double diffusion method and the immunoelectroosmophoresis (IEOP) was made. The IEOP technique using pure agarose and staining the plates was 21 times more sensitive than the Ouchterlony method. In cases of hepatitis it was possible to detect Australia antigen for 50% longer time with IEOP than with the Ouchterlony method. The practical application of IEOP for detection of Australia antigen and antibodies is discussed.