Spectrophotometric Estimation of Glibenclamide

摘要

Two simple and sensitive Spectrophotometric methods are developed for the Quantitative estimation of glibenclamide in pure and pharmaceutical formulations.Both methods are based on the formulation of coloured oxidative coupling product with p-N,N-dimethyl phenylene diamine(DMPD)and chloramine-T(CAT)or 2,6-dichloroquinone chlorimide(DCQC)exhibiting maximum absorption at 535 and 530 nm respectively.Glibenclamide(GBC)1 is an antidiabetic agent and belongs to the class sulfonyl urea.Chemically it is 10[4-[2-(5-chloro-2-methoxy benzamide ethyl] benzene sulphonyl]-3-cyclohexyl urea.A few methods were reported for the estimation of GBC.The methods so far reported are HPLC_2,UV3 and colorimetry.