Direct Photometric Determination of Lead by Manual and Flow Injection Methods with Gallocyanine

摘要

A direct photometric method was developed for the determination of sub-nanogram levels of lead. The method is based on complexation between lead and gallocyanin,which form a coloured dye (λ_(max) 550 nm) at pH 8.0. The determination was based on the use of kinetic approach and in the lead concentration range of 1.0×10~(-3) μg/mL to 1.0×10~1 μg/mL,a smooth calibration curve was obtained. The reaction system can also be successfully adapted to flow injection analysis (FIA). The dynamic range of the proposed flow injection method was 1.0×10~3 μg/mL to 1.0×10~2 μg/mL and detection limit was 1.6 ng/mL at a sampling rate of 30 injections per hour. At 1:1 mole ratio of lead to the interfering ion,mercury,iron,aluminium,citrate and fluoride were found to interfere most during the determination.