Simultaneous Spectrophotometrie Estimation of Nebivolol Hydrochloride and Hydrochlorothiazide in Tablets

摘要

Two simple, accurate and precise methods for simultaneous estimation of nebivolol hydrochloride (NEB-H) and hydrochlorothiazide (HCT) in combined dosage form have been described. First method employs formation and solving of Q-absorbance equation at 275 nm (isoabsorptive point) and at 282.5 nm (λ_(max) of NEB-H). The second method involves formation and solving of simultaneous equation using 282.5 and 271.5 nm (λ_(max) of HCT) using methanol as solvent. The result of analysis has been validated statistically and by recovery studies with standard deviation < 1.0 % was found. The proposed methods were successfully applied for estimation of nebivolol hydrochloride and hydrochlorothiazide in combined tablet formulation.