HPLC Determination of 5-Fluorouracil in Human Plasma

摘要

The present study describes the HPLC method for the estimation of 5-fluorouracil in human plasma. Varying amounts of 5-fluorouracil (10 to 400 ng) were added to drug-free human plasma (0.5 mL) and mixed with 0.3 mL of acetonitrile. The mixture was vortexed for 5 min and centrifuged at 3, 000 rpm for 10 min and the supernatant liquid was filtered. Twnety microlitres of the resultant filtrate was injected into a reverse phase C-18 column using a mobile phase consisting of HPLC grade water at a flow rate of 0.8 mL/min. The eluents were monitored at 260 nm. On conducting validation study, it was found that the method is simple, precise, specific, les time consuming and accurate for the estimation of 5-fluorouracil in human plasma samples.