Rapid Determination of Four Tobacco-Specific Nitrosamines in Mainstream Cigarette Smoke by UPLC-TOF-MS

摘要

A rapid method for determining four major tobacco-specific nitrosamines (TSNAs) in mainstream cigarette smoke based on ultra-performance liquid chromatography coupled to time-of-flight mass spectrometry (UPLC-TOF-MS) has been developed. Cigarette smoke particulate, collected on a Cambridge filter pad, was extracted by using ammonium acetate buffer as solvent, purified by solid phase extraction (SPE) and analyzed by UPLC-TOF-MS with positive electrospray ionization (ESI~+). The method has shown excellent sensitivity, repeatability and accuracy.