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Assessment of molecular divergence using microsatellite markers linked to drought-yield QTLs of rice (Oryza sativa L.)

机译:使用与水稻干旱产量QTL相关的微卫星标记评估分子分化(Oryza sativa L.)

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摘要

The drought stress is the major limitation for the production and yield stability in the rice (Oryza sativa L.) growing areas particularly under rainfed conditions. The assessment of genetic diversity among the rice genotypes using molecular markers linked to drought-yield QTLs will help in the selection of divergent parents to produce superior recombinants and transgressive segregants to develop high yielding cultivars for drought conditions. So the current study was conducted to estimate the genetic diversity among the set of twenty four advanced drought tolerent rice genotypes and commercial checks using SSR markers linked to major drought-yield QTLs. A total of 31 alleles were detected by 13 polymorphic markers across 24 rice genotypes with a mean of 1.94 alleles per polymorphic marker. Among the polymorphic markers, II markers formed 2 alleles each, 2 markers have produced 3 alleles each. The PIC value observed in the contemporary study ranged from 0.239 to 0.499 with an average PIC value of0.346. The dendrogram displayed a total five clusters on the basis of dissimilarity coefficient values, the cluster II had maximum ten genotypes followed by cluster IB-a with five genotypes and clusters IA-a, IA-b, IB-b with three genotypes each. IR 82475-110-2-2-1-2 and IR92937-178-2-2 (R-155) were found to be the most divergent genotypes.
机译:干旱胁迫是水稻(Oryza sativa L.)种植区生产和产量稳定性的主要制约因素,特别是在雨养条件下。使用与干旱产量QTL相关的分子标记评估水稻基因型之间的遗传多样性将有助于选择不同的亲本,以产生优良的重组体和越界的分离体,以开发适合干旱条件的高产品种。因此,本研究旨在评估24个高级耐旱水稻基因型的遗传多样性,并使用与主要干旱产量QTL相关的SSR标记进行商业检查。在24个水稻基因型中,13个多态性标记共检测到31个等位基因,平均每个多态性标记1.94个等位基因。在多态性标记中,II标记各形成2个等位基因,2个标记各产生3个等位基因。在当代研究中观察到的PIC值范围为0.239至0.499,平均PIC值为0.346。树状图在相异系数值的基础上共显示5个簇,簇II最多有10个基因型,其次是5个基因型的IB-a簇和IA-a、IA-b、IB-b簇,各有3个基因型。IR 82475-110-2-2-1-2 和 IR92937-178-2-2 (R-155) 被发现是差异最大的基因型。

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