A New Method of Discrimination for Peaks of Enantiopure Compounds in Chiral Separation of HPLC

摘要

Diode array detector is one of the most popular detectors of high performance liquid chromatography (HPLC). With the full-band scanning, it has been used extensively for the qualitative and quantitative analysis of components. In this paper, a few groups of enantiomers, diastereomers, isomers and homologue were chosen as analytes and a new method of discrimination for their peaks in chromatogram of HPLC was tried to be developed. By using diode array detector, we obtained peak characteristics for them. By comparing chromatograms of enantiomers, diastereomers, isomers and homologue, we find that only the chromatograms of the enantiomers and diastereomers have considerable consistency. Then a new method of discrimination for peaks of enantiopure compounds by diode array detector has been established. This method can be used for research of chiral separation.