Quantification of beta-Sitosterol and Puerarin from Pueraria tuberosa DC.by Using High Performance Thin Layer Chromatography

摘要

Sensitive,simple and accurate high-performance thin-layer chromatographic methods have been established for determination of beta-sitosterol and puerarin in root powder of Pueraria tuberosa DC.Methanolic extracts of root powder were used for the experimental work.beta-Sitosterol and puerarin were found to be 0.03 and 2.45 %,respectively,in root powder of Pueraria tuberosa DC,by the proposed methods.Separation was performed on aluminium-backed silica gel 60 F_(254)HPTLC plate for both the standards.Detection and quantification were performed by densitometry at lambda=257 nm and lambda=366 nm for puerarin and p-sitosterol,respectively.Puerarin response was linear over the range 20 to 80 mu g mL~(-1)where as P-sitosterol response was linear over the range 10 to 60 mu g mL~(-1).The validated HPTLC methods can be used for a routine quality control analysis of Pueraria tuberosa DC.and quantitative determination of puerarin and beta-sitosterol.