HPLC Separations of Normal and Modified Nucleobases and Nucleosides on an Amino Silica Gel Column

摘要

An amino substituted silica gel column was used for the normal phase HPLC separation of nucleic acid components, including free bases and the corresponding 2′-deoxyribo- and ribonucleosides. The chromatographic system was also found to be particularly convenient for the separation of various modified nucleosides, including oxidation products of 2′-deoxyguanosine and pyrimidine components, thymidine cyclobutane dimers, dinucleoside monophosphate photoproducts and pyrimidine (6-4) pyrimidone adducts.