Determination of Natural Occurrence of Ochratoxin A in Naked Oats of China by HPLC

摘要

Ochratoxin A is a ubiquitous mycotoxin produced by different species of Aspergillus and Penicillium fungi. It is widespread in food and feed and its occurrence has been reported in cereals, cereal-derived products, dried fruits and spices. The aim of our study was to investigate the presence of ochratoxin A in naked oats which was widely consumed and commercialized in China. The analytical methods used in this study involved the extraction of ochratoxin A by acidified toluene, immunoaffinity (IAC) clean-up and high performance liquid chromatography (HPLC) quantification with fluorescence detection. The limit of detection for ochratoxin A was 0.5 μg kg~(-1). Levels and percentages of ochratoxin A contamination in different types of naked oats, 35 white oats, 56 upon dam naked oats and 48 Mongolia naked oats samples, were evaluated with incidences of 31, 23 and 33 %, respectively. The average of contamination by ochratoxin A found were 3.85, 2.33 and 2.38 μg kg~(-1), respectively, for white oats, upon dam naked oats and Mongolia naked oats. Experimental results showed that the contamination percentages and levels in 2010 were lower than usual norms (5 μg kg~(-1)) established by the European commission in 2002.