Reversed-Phase HPLC Method for Determination of Quercetin in Human Plasma

摘要

A simple reversed-phase liquid chromatography method was developed for the quantitative determination of quercetin.The method is simple,sensitive and highly selective and involves single extraction of drug from plasma in(4:1)ratio of methanol:DMSO.The mobile phase was pumped at a flow rate of 1.0 mL/min and the effluent was monitored at 370 nm.The retention time of quercetin was 2.72 min.The limit of detection of drug in plasma was found to be 0.2 mu g/mL.The method was validated for its linearity,precision and accuracy.