Simultaneous Quantitative Determination of Gentiana scabra by HPLC-MS/MS

摘要

An approach for simultaneous quantitative determination of bioactive components in traditional Chinese medicine, using HPLC-MS/MS technique was described in this paper. Gentiana scabra, a well-known traditional Chinese medicine, was studied using the established method as for an application. The column was Rromasil C18 and the gradient mobile phase was optimized for a better resolution. As a result, the linearities and recoveries of the 6 components were suitable and good for the detection. This work could provide a simple, rapid and relatively comprehensive quality control method for the clinical safety of G. scabra.