Demineralization with EDTA by constant replacement

摘要

AbstractAn apparatus for demineralization of hard tissues by constant replacement of a saturated solution of EDTA is described. This method allows for more rapid demineralization through better Ca chelation. EDTA demineralization preserves cells and matrix constituents particularly the mucopolysaccharides as demonstrated by staining, Alpharadiography, phase contrast microscopy and microincineration. Cold acetone fixation followed by cold EDTA demineralization retains several enzymic activities.