Effect of glycinin and beta -conglycinin on the absorbing capacity of mouse intestinal epithelial cells.

摘要

Glycinin and beta -conglycinin in soybeans can reduce the absorption of nutrients by young animals and result in watery diarrhea. In this study, self-prepared purified glycinin and beta -conglycinin were applied to intestinal epithelial cells of primary cultured mice. The effects of purified glycinin and beta -conglycinin (0.1, 5 and 10 mg mL-1) on Na+-K+-ATP enzyme, PEPT1 and DMT1 expression in mouse intestinal epithelial cells were studied. Glycinin and beta -conglycinin could enhance epithelial cell Na+-K+-ATP enzyme activity but reduced the amount of PEPT1 and DMT1. The effect of beta -conglycinin was more pronounced than that of glycinin.