Haematological and Biochemical Alterations in Calves Following Infection with Pasteurella multocida Type B: 2, Bacterial Lipopolysaccharide and Outer Membrane Protein Immunogens (OMP)

摘要

Pasteurella multocida is an opportunistic pathogen with veterinary and human significance. The species diverse and complexity between the types exist with respect to antigenic variation, host predilection and pathogenesis. The aim of the present studywas to determine hematological and serum concentration of biochemical values in calves having infected with Pasteurella multocida type B and its immunogens; Lipopolysaccharide (LPS) and Outer Membrane Protein (OMP). A total of eight clinically healthy calves were divided into four groups of 2 calves in each group. Group 1 were inoculated with sterile Phosphate Buffered Saline (PBS). Group 2 were inoculated with wild-type P. multocida type B: 2 while Group 3 and 4 were inoculated with LPS and OMP extracted from Pasteurella multocida type B, respectively. Blood samples were collected for haematological and biochemical analysis. Results indicated significant differences in the mean values of Prothrombin Time (PT), Partial Thromboplastin Time (PTT) and thrombocyte levels in infected groups 2-4 compared to the control group. There was significant decrease in the mean concentration of Alanine Transaminase (ALT) for group 2 (20.607+-1.639 U L~(-1)), group 3 (22.067+-1.617 U L~(-1)) and group 4 (40.327+-8.351 U L~(-1)). Conversely, there was no significant difference in Alkaline Phosphatase (ALP) for group 2 (149.857+-17.327 U L~(-1)) but a significant decrease for groups 3 (114.147+-20.901 U L~(-1)) and group 4 (128.97U17.068 U L~(-1)). There was no significant differences in the serum levels of Gamma-glutamyltranspeptidase (GGT) and almost all tested electrolytes in infected groups. In conclusion, our results provide evidence in support of using some haematological and biochemical markers for early detection of individuals at risk for wild type of Pasteurlla multocida.