The muscle transcription factor MyoD regulates the expression of hepatocyte growth factor during the process of muscle development.

摘要

Muscle regeneration recapitulates myogenesis. HGF (hepatocyte growth factor) plays an important role in muscle development, as the only secreted growth factor, which can activates myoblasts proliferation. The expression units of wild HGF promoter-luciferase vector exceeded the expression units of HGF promoter-luciferase vector with mutated binding sequence of MyoD, also the expression trend of HGF was in accordance with the expression of MyoD. The expression of MyoD directly affects the expression of HGF during muscle development. In C2C12 culture, the expression of HGF is up-regulated in the phase of proliferation process, but down-related in the phase of differentiation. We identified the proliferation and differentiation stage of C2C12 culture by the definition of expression of myogenic regulator family (MyoD, yf5, yogenin, Myf6) and detected the HGF level during this two stages. MyoD bind site was found in the 5'-UTR of HGF promoter. MyoD is b HLH (basic helix-loop-helix) transcriptional activator which has a critical role during myogenesis. We verified that MyoD can bind the active b HLH site, as shown by EMSA. Transfection of wild HGF promoter-luciferase eukaryotic expression vector with MyoD binding site and HGF promoter-luciferase eukaryotic expression vector with mutated MyoD binding site into C2C12 myoblasts was done.