Supplementation Vitrified-thawed Media with Melatonin Do Not Protecting Immature Mouse Testicular Tissue from Vitrified-thawed Induced Injury

摘要

The purpose of this study was to test of efficacy of melatonin on the Optimizing of cryopreservation media in the testis tissue samples. Testes from neonate BALB/c mice were vitrified and then thawed under standard condition with or without the addition of 100 mu M melatonin to both of vitrification and thawing solution. After that, Vitrified-thawed whole testes were digested under standard condition and subsequent viability of the cells in the suspension was analyzed using cytotoxicity kit and Apo-Brdu tunnel assay kit. The mean proportion of apoptotic testicular cells in the treated vitrified-thawed testes in comparison to no-treated ones was noted significantly (5.2±0.47 vs. 1.56±0.62, respectively). Moreover, melatonin cause decreasing the viability of the treated vitrified-thawed testicular cells in compared to no-treated vitrified-thawed testicular cells (4.78±0.46 vs. 8.39±0.76, respectively). In addition, the mean cytotoxicity of melatonin on the vitrified-thawed testicular cells was 9%. The associated reduction in healthy testicular cells in the treated vitrified-thawed testes suggests that melatonin in doses of 100 nu M don't protected testicular tissue from damaged induced in the process vitrification and thawing. However, further well-designed studies such as dosimetry melatonin and applied another cryoprotectants in the matching with melatonin are essential to offer a final conclusion.