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Introgression lines of rice (Oryza sativa L.) carrying a donor genome from the wild species, O. glumaepatula Steud. and O. meridionalis Ng.

机译:携带来自野生物种 O的供体基因组的水稻(Oryza sativa L.)的渗入系。 glumaepatula Steud。和 O。子午线

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To broaden the available rice genetic resources, we developed two populations of introgression lines of cultivated rice (Oryza sativa) carrying donor segments from the wild species Oryza glumaepatula and Oryza meridionalis. These lines contain overlapped introgressed donor segments that covered most parts of the genome of the two donors in the same of O. sativa genetic background (ssp. japonica, cv. Taichung 65). The introgression lines were developed through repeated backcrossing with Taichung 65 as a pollen parent and marker assisted selection. O. glumaepatula introgression lines consist of two sets of the lines (with O. glumaepatula and Taichung 65 cytoplasm, respectively): these comprise a total of 69 lines that cover 79.5 to 89.2% of the Oryza glumaepatula genome. The O. meridionalis introgression lines also consist of two sets of the lines (with O. meridionalis and Taichung 65 cytoplasm, respectively): these comprise a total of 78 lines covering the 81.5 to 98.0% of the Oryza meridionalis genome. These introgression lines significantly broaden rice genetic resources, and will facilitate analyses of the genetics of traits specific to the donor species.Digital Object Identifier http://dx.doi.org/10.1270/jsbbs.60.597
机译:为了拓宽可利用的稻米遗传资源,我们开发了两个栽培稻( Oryza sativa )渗入系,它们带有来自野生物种 Oryza glumaepatula 和 Oryza的供体片段。子午线。这些品系包含重叠的渗入供体片段,这些片段在相同的iO中覆盖了两个供体的基因组的大部分。苜蓿的遗传背景(sp。 japonica ,cv。Taichung 65)。通过与作为花粉亲本的台中65和标记辅助选择进行反复回交来形成基因渗系。哦glumaepatula 基因渗入系由两组细胞系组成(分别具有 O。glumaepatula 和台中65细胞质):这些共包含69个细胞系,覆盖了79.5至89.2%的细胞系。 i.Oryza glumaepatula 基因组。 O。子午线基因渗入系还包括两组子线(分别具有子午线虫和台中65细胞质):这些共包括78株,覆盖了81.5%到98.0% 子午线基因组。这些基因渗入系显着拓宽了水稻的遗传资源,并将有助于分析供体物种特有的性状。数字对象标识符http://dx.doi.org/10.1270/jsbbs.60.597

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