Cloning and sequencing of full-length cDNAs of RNA1 and RNA2 of a Tomato black ring virus isolate from Poland.

Jonczyk M; Le Gall O; Palucha A; Borodynko N; Pospieszny H

首页> 外文期刊>Archives of virology >Cloning and sequencing of full-length cDNAs of RNA1 and RNA2 of a Tomato black ring virus isolate from Poland.

【24h】

Cloning and sequencing of full-length cDNAs of RNA1 and RNA2 of a Tomato black ring virus isolate from Poland.

机译：波兰番茄黑环病毒分离株RNA1和RNA2全长cDNA的克隆和测序。

获取原文

获取原文并翻译 | 示例

获取外文期刊封面封底 >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Full-length cDNA clones corresponding to the RNA1 and RNA2 of the Polish isolate MJ of Tomato black ring virus (TBRV, genus Nepovirus) were obtained using a direct recombination strategy in yeast, and their complete nucleotide sequences were established. RNA1 is 7358 nucleotides and RNA2 is 4633 nucleotides in length, excluding the poly(A) tails. Both RNAs contain a single open reading frame encoding polyproteins of 254 kDa and 149 kDa for RNA1 and RNA2 respectively. Putative cleavage sites were identified, and the relationships between TBRV and related nepoviruses were studied by sequence comparison.

机译：使用直接重组策略在酵母中获得了与番茄黑环病毒波兰分离株MJ（TBRV，Nepovirus属）的波兰分离株MJ对应的全长cDNA克隆，并建立了其完整核苷酸序列。 RNA1的长度为7358个核苷酸，RNA2的长度为4633个核苷酸（不包括poly（A）尾巴）。这两个RNA都包含一个单一的开放阅读框，分别编码RNA1和RNA2的254 kDa和149 kDa的多蛋白。鉴定了假定的切割位点，并通过序列比较研究了TBRV与相关神经病毒之间的关系。

著录项

来源
《Archives of virology》 |2004年第4期|共9页
作者
Jonczyk M; Le Gall O; Palucha A; Borodynko N; Pospieszny H;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
RNA I; Length; Tomatoes; Virus; Nucleotides; 番茄; 核苷酸类;

机译：RNA I;Length;Tomatoes;Virus;Nucleotides;番茄;核苷酸类;

相似文献

外文文献
中文文献
专利

1. Cloning and sequencing of full-length cDNAs of RNA1 and RNA2 of a Tomato black ring virus isolate from Poland. [J] . Jonczyk M, Le Gall O, Palucha A, Archives of virology . 2004,第4期

机译：波兰番茄黑环病毒分离株RNA1和RNA2全长cDNA的克隆和测序。
2. Construction of full-length cDNA clones to Soil-borne wheat mosaic virus RNA1 and RNA2, from which infectious RNAs are transcribed in vitro: Virion formation and systemic infection without expression of the N-terminal and C-terminal extensions to the [J] . Yamamiya A., Shirako Y. Virology . 2000,第1期

机译：构建土壤传代的小麦花叶病毒RNA1和RNA2的全长cDNA克隆，从中转录出感染性RNA：病毒体形成和系统感染，而没有表达N端和C端延伸
3. The sequencing of the complete genome of a Tomato black ring virus (TBRV) and of the RNA2 of three Grapevine chrome mosaic virus (GCMV) isolates from grapevine reveals the possible recombinant origin of GCMV [J] . Digiaro M., Yahyaoui E., Martelli G. P., Virus Genes . 2015,第1期

机译：番茄黑环病毒（TBRV）的完整基因组序列和葡萄中三种葡萄铬花叶病毒（GCMV）分离株RNA2的测序揭示了GCMV的可能重组来源
4. An Integrated Database System for Full-Length Human cDNA in NEDO Full-Length Human cDNA Sequencing Project [C] . Tetsuo Nishikawa, Kouichi Kimura, Tomohiro Yasuda, Workshop on Genome Informatics . 2002

机译：NEDO全长人cDNA测序项目全长人cDNA集成数据库系统
5. Generation of full-length cDNA clone and functional analysis of leader proteases of Grapevine leafroll-associated virus-2. [D] . Liu, Yu-Ping. 2009

机译：葡萄叶片相关病毒2的全长cDNA克隆的产生和前导蛋白酶的功能分析。
6. Comparison of the 5′ and 3′ termini of tomato ringspot virus RNA1 and RNA2: Evidence for RNA recombination [O] . M.E. Rott, J.H. Tremaine, D.M. Rochon -1

机译：番茄环斑病毒RNA1和RNA2 5和3末端的比较：RNA重组的证据
7. Construction of Full-Length cDNA Clones to Soil-Borne Wheat Mosaic Virus RNA1 and RNA2, from Which Infectious RNAs Are Transcribed In Vitro: Virion Formation and Systemic Infection without Expression of the N-Terminal and C-Terminal Extensions to the Capsid Protein [O] . Yamamiya Aya, Shirako Yukio 2000

机译：土生小麦花叶病毒RNA1和RNA2全长cDNA克隆的构建，可从中转录感染性RNA：病毒体形成和系统感染，不表达衣壳蛋白的N末端和C末端延伸

Cloning and sequencing of full-length cDNAs of RNA1 and RNA2 of a Tomato black ring virus isolate from Poland.

摘要

著录项

相似文献

相关主题

期刊订阅