Determination of Flurbiprofen in Dosage form and in Biological Fluids by High Performance Liquid Chromatography

摘要

A high performance liquid chromatograpy method is described for the determination of flurbiprofen in both dosage form and in biological fluids (urine and plasma) using fluorescence detection. The method for dosage forms involves grinding of a 100 mg tablet, suspension in methanol, filtration and adjusting to the appropriate concentration and 10 ul is injected onto the column. In the case of biological fluids a series of standard solutions were prepared in 0.IN sodium hydroxide and a known amount was added to 1 ml of serum or urine which was then acidified, extracted with ethylacetate, evaporated and the residue was then dissolved in a known volume of the mobile phase. Complete separation of the drug was achieved in about 6.5 minutes under the used conditions.