STUDIES ON THE METABOLISM OF A SULFUR-OXIDIZING BACTERIUM II. THE SYSTEM OF CO2-FIXATION INTHIOBAC1LLUS THIOOXIDANS

摘要

In the early stage of CO2-fixation byThiobacillus thiooxidans, which was incubated aerobically in the presence of sulfur, most part of the fixed carbon was found in the phosphate ester fraction.The fixation was inhibited by NaF, picolinic acid, PCMB, azide, dipyridyl,o-phenanthroline, monoiodoacetic acid, and arsenite, each in the concentration range where the sulfur oxidation was not affected strongly.The crude extract of this organism could fix CO2in the presence of ATP, R-5-P and Mg++. Most part of the fixed carbon was found in PGA.The crude extract showed the CO2-fixation coupled with the H2S-oxidation in the presence of ADP.An appreciable reduction of PGA could not be detected in the presence of reducing systems, involving TPNH and DPNH.