Evaluation of maize genotypes against downy mildew (Peronosclerospora sorghi (Weston and Uppal) and characterization of soluble seed proteins by SDS-PAGE

摘要

In this investigation, four local inbred maize (Zea mays L.) genotypes CM-500, NAI-127, NAI-129 and SKV-10 were evaluated in the field against Peronosclerospora sorghi during two seasons, i.e., Kharif and Rabi, 2005. The results indicate that genotype SKV-10 was found highly resistant to downy mildew recording 1.0% of disease during both seasons. Whereas NAI-129 had low incidence (10.2 and 13.0% respectively for Kharif and Rabi) of downy mildew compared to CM-500 (97.2 and 99.4% respectively for Kharif and Rabi). The characterization of soluble seed proteins by SDS-PAGE indicates CM-500 as highly susceptible to downy mildew and revealed considerable variation among the genotypes. It is evident from the dendrograms that CM-500 and NAI-127, as well as NAI-129 and SKV-10, were genetically closely related, consistent with the compatible and incompatible reaction of these genotypes to downy mildew.