ANTIVIRAL ACTION OF HARPULIA CUPANIOIDES AND MIRAB1L1S JALAP A AGAINST TOMATO SPOTTED WILT VIRUS (TSWV) INFECTING TOMATO

摘要

Mirabilis jalapa and Harpulia cupanioides remain as an unexploited potential to be tapped, as sources of antiviral principles. The antiviral protein from M. jalapa (MAP) and H. cupanioides (HAP) were highly effective in inhibiting TSWV at 60 and 80% saturation, A minimum concentration of 400 fig/ml of MAP was sufficient to inhibit TSWV. HAP at 800 μg/ml recorded 98,41% inhibition of lesion formation. The plants treated with M. jalapa and H. cupanioides challenged with TSWV did not show significant variation in absor bance values when compared to uninoculated healthy plants, indicating the absence of detectable virus through indirect DAS ELISA thus indicating the effectiveness of AVPs. Pie-application of MAP and HAP induced the activity of phenols,PO, PPO and PAL leading to the suppression of TSWV on local lesion and systemic host.