Genetic analysis of geminiviral DNA from tomato yellow leaf curl diseased tomato (Lycopersicon esculentum Mill.) plants from Tanzania

摘要

DNA was extracted from tomato plants showing symptoms of tomato yellow leaf curl disease. Using degenerate primers PAClvl978 and PAVlc715, a putative viral fragment of approx 1.2 kb was amplified from the extracted DNA. The amplified viral DNA was isolated, cloned into Psrl-digested pBluescript KS~ + plasmid and sequenced. Sequence comparison indicated that the virus causing tomato yellow leaf curl disease in Tanzania was genetically homologous (97% identity in the coat protein) to Tomato leaf curl Tanzania virus. There were no significant differences in DNA sequences among the field isolates from Tanzania.