Effect of salicylic acid and methyl jasmonate on phenylalanine ammonia-Iyase activity and total phenol in wheat infected by Pratylenchus thornei

摘要

The effect of biochemical responses in wheat seedlings cultivar Falat against lesion nematode (Pratylenchus thornei) was investigated by two factors inducer resistance salicylic acid (SA) and methyl jasmonate (MeJA). Foliar sprays with 1 ml SA 1 mM and 1 ml MeJA 100 uM was conducted on 20-day-old wheat (cv. Falat) seedlings. About 24 h after foliar sprays, plants were inoculated by root lesion nematode. Phenylalanine ammonia-lyase (PAL) activity was increased significantly in all treatments two daysafter inoculation over control. However, PAL activity was decreased in all treatments five days after inoculation in compared to control. What is more, in both time points, total phenol increased in all treatments over control. The comparison of both inducers SA and MeJA on total phenol in healthy and infected wheat seedlings in both days two and five after inoculation of nematode indicated that total phenol has decreased significantly in all treatments in the fifth day.