Identification of Soybean mosaic virus and Zucchini yellow mosaic virus isolated from Iran and phylogenetic comparison with other isolates

摘要

As the important resource in human nutrition, vegetables are susceptible to several viruses. Potyvirus as the most important genus in the family Potyviridae causes severe crop yield reduction. We tried to detect a genomic fragment, amplified by RT-PCRby use of the Potyvirus degenerate primers (NIb2F/Nlb3A), from samples of vegetables in East Azerbaijan. A pTZ57R/T vector was prepared by adding thymine nucleotide to the free 5' end of the linear plasmid, to clone the fragments. Recombinant plasmids were transformed in Escherichia coli as a prokaryotic host. To investigate the identity of genomic fragments, sequencing was carried out and finally, some genomic segments were investigated to be SMV and ZYMV that are membranes of Potyvirus genus. This isthe first report of ZYMV from this region of Iran based on RT-PCR detection and SMV is sequenced for first time from Iran. Finally, phylogenetic comparison with other isolates was carried out for these two isolate of Potyvirus genus.