Crude honey bee venom and Bacillus thuringiensis L. Crylc toxin in controlling wax moth Achroia grisella F. (Lepidoptera: Pyralidae)

摘要

Achroia grisella, is a noxious pest of honey bee hives. In this study the toxicity of honey bee venom and bacterial CrylC toxin of Bacillus thuringiensis on A. grisella were assessed. In addition, the synergistic interaction between BT Crylc and crudehoney bee venom was determined. The combination of HBV and CrylC increased the activity for both toxins. Therefore, further analysis was carried out to assess the synergistic interaction of each compound assay separately and in mixture. The results showed LC50 value of 1.105 u.g/p.1 for HBV and LC50 value of 0.201 ug/u.1 for CrylC. The combination bioassay result showed an LC50 value of 0.549 p.g/u.1. The combined calculated LC50 is containing lower concentration of the component toxins, this result proved that the toxicity was enhanced due to the combination. A chi square value of 39.93and relative synergistic factor ratio of 1.14 confirmed that a synergic interaction was achieved in combination.