Glycerylalkylsilylated Silica Gels for Direct Injection Analysis of Drugs in Serum by High-Performance Liquid Chromatography

摘要

A new packing material for direct: injection analysis of drug in serum by high-performance liquid chromatography is described. It was found out that proteins are no: adsorbed but low-molecular-weight compounds are analyzed by reversed-phase high-performance liquid chromatography using glycerylalkylsilylated silica gels with alkyl moiety ranging from C3 to C6. On chromatography using glycerylundecylsilylated silica gel, on the other hand, retention of low-molecular-weight compounds increased but recovery of proteins decreased from it. Reversed-phase separation of drugs on the new packing materials by direct injection of serum samples is demonstrated.