Oxalic acid production by Fusarium oxysporum Schlecht and Botryodiplodia theobromae Pat., post-harvest fungal pathogens of yams (Dioscorea rotundata L.) and detoxification by Bacillus subtilis CM1 isolated from culturable cowdung microflora

摘要

Fusarium oxysporum Schlecht and Botryodiplodia theobromae Pat., two important post-harvest pathogens of yam (Dioscorea rotundata L.) tubers in storage were found to produce oxalic acid (OA) in vitro and in vivo. The rate of OA accumulation was proportional to fungal growth (cell mass) in Potato Dextrose liquid medium during 10 days incubation period. Further, simultaneous co-culturing of either of the fungi with Bacillus subtilis CM1 isolated from cowdung culturable microflora resulted in 92% reduction in OA accumulation compared with that in the culture of the individual fungus. The effect was more prominent in pH 5-6 than in pH 7-8. B. subtilis CM1 was capable of detoxifying OA and several proteins were detected in the culture filtrates when it was grown in peptone-mineral salt medium containing OA. SDS-PAGE analysis of 70% ammonium sulphate fraction of the culture nitrate exhibited the presence of a predominant 97 kDa protein.