A new furostanol saponin, (25S)-26-O-beta-D-glucopyranosyl-5beta-furost-20(22)-en-3beta, 15beta,26-triol-3-O-[alpha-L-rhamnopyranosyl-(1-4)]-beta-D: -glucopyranoside, namely, aspacochioside D (1) were isolated from Asparagus cochinchinensis (Lour.) Merr, along with three known saponins, aspacochioside C (2), (25S)-5beta-spirostan-3beta-yl-O-[O-alpha-L-rhamnopyranosyl-(1-4)]-beta-D-glucopy ranoside (3), and pseudoprotoneodioscin (4). The structure of 1 was elucidated on the basis of chemical reactions and spectral analysis (IR, GC, ESI-MS, (1)H-NMR, (13)C-NMR, DEPT, HMBC, HMQC and NOESY). The antiproliferative effects of 1-4 were evaluated in a cytotoxicity assay against the human tumor cell line, A549. Compound 2 (Aspacochioside C) exhibited moderate cytotoxicity against A-549, with an IC(50) value of 3.87 mug/mL.
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