Preparative Scale High-Performance Liquid Chromatography and Identification of Cyclosporine-A from an Indigenous Fungal Isolate

摘要

Extract of fermentation broth of an indigenous funqal isolate of Tolypocladium sp., found to contain Cyclosporine-A (Cy A) has been investigated by spectroscopic and immunological analyses. A preparative scale High Performance Liquid Chromatogrphy (HPLC) for Cy A on an analytical reversed phase HPLC column by interval injection followed by gradient elution has been described. The identity of this preparation was confirmed by matching the spectra with the reference standard using photo diode array detector.