Functional analysis of Trypanosoma congolense P74 gene by double strand RNA interference.

摘要

Trypanosoma congolense RNA interference cell lines were used to determine function of Trypanosoma congolense P74 gene by double strand RNA interference. The present study strongly indicates and further verified the data of the previous research that,P74 gene is stage-specific and expression is developmentally up-regulated in Trypanosoma congolense bloodstream forms. The inability to determine the function due to the stage-specific expression of the gene still contributed to the understanding of thenature of the gene and double strand RNA interference in trypanosomes. The study further stress that T. congolense is still amenable to double strand RNA interference and is an excellent model species to conduct laboratory studies in stage-specific geneexpression.