Descriptive analysis of the draft genome from the melanin?producing bacterium Sinorhizobium (Ensifer) meliloti CE52G

摘要

Herein we present the draft genome (7.32 Mb and 62 GC content) of melanin- and laccase-producing Sinorhizobium (Ensifer) meliloti strain CE52G isolated from alfalfa nodules. We analyzed the genome and, performed a few in-bench experiments to corroborate some information. We found the gene for the laccase, probably involved in the production of the pigment melanin. CE52G shows genes for: (i) Embden-Meyerhof (EMP) and Entner-Doudoroff (ED) pathways; (ii) dissimilation and assimilation of nitrate, assimilation to ammonia, and conversion of nitrous oxide to nitrogen; (iii) Nodulation Factor and Mo-nitrogenase synthesis; (iv) synthesis and degradation of polyhydroxybutyrate and glycogen; (v) succinoglycan (EPS I) and galactoglucan (EPS II); and (vi) acidic and oxidative stress endurance. This bacterium does not produce siderophores but has the genomic machinery for the acquisition of siderophores (produced by other organisms) and hemophores for the uptake of heme from the environment. Among others, we found genes for two-component systems involved in the uptake of dicarboxylic acids and, the response to acidic conditions. The general information is discussed in terms of their relevance during CE52G association with plants and environmental adaptation.