Enhancing DNA sequencing

摘要

Next-generation sequencing (NGS) revolutionized the biological sciences and genomic research in particular by providing a fast and scalable ultra-high throughput method. Hereby, genomes are usually fragmented into small pieces to facilitate their sequencing in a single experimental setup. The ends of the fragments need to be repaired, whereby T4 DNA polymerase (T4 pol) and T4 polynucleotide kinase (T4 PNK) are typically used. For the subsequent DNA modification step, these enzymes are generally heat inactivated, which is assumed to negatively affect the sequencing of AT-rich regions.