Molecular Cloning and Characterization of a cDNA Encoding Cytochrome c Oxidase Subunit Va From the Lesser Grain Borer,Rhyzopertha dominica (F.)(Coleoptera:Bostrichidae)

摘要

A cDNA encoding subunit Va of cytochrome c oxidase (EC 1.9.3.1)was cloned and characterized froma lesser grain borer (Rhyzopertha dominica)cDNA library.The complete cDNA consists of 693-bp and contains an open reading frame of 450-bp that encodes 150 amino acid residues.The seuence includes a 28-bp putative N-terminal and a 122-bp putative mature protein.The estimated moleuclar weight and pl for the predicted mature protein are 13,962 and 4.60,respectively.The cDNA-deduced amino acid sequence of the mature protein shows 73% identity to that of a corresponding subunit of African malaria mosquito (Anopheles gambiae)and 59% identity to that of the fruit fly (Drosophila melanogaster).In addition,31% of all amino acid residues are conserved among six different animal species.Evolutionary distance analysis suggests that cytochrome c oxidase subunit Va from R.dominica is most similar to the corresponding subunit from the malaria mosquito.Northern analyssi revealed a single 4.9-kb transcript that is much larger than that found in mammalian species.