AhR modulates the IL-22-producing cell proliferation/recruitment in imiquimod-induced psoriasis mouse model

摘要

IL-22 has a detrimental role in skin inflammatory processes, for example in psoriasis. As transcription factor, AhR controls the IL-22 production by several cell types (i.e. Th17 cells). Here, we analyzed the role of Ahr in IL-22 production by immune cells in the inflamed skin, using an imiquimod-induced psoriasis mouse model. Our results indicate that IL-22 is expressed in the ear of imiquimod-treated Ahr(-/-) mice but less than in wildtype mice. We then studied the role of AhR on three cell populations known to produce IL-22 in the skin: gamma delta T cells, Th17 cells, and ILC3, and a novel IL-22-producing cell type identified in this setting: CD4(-)CD8(-)TCR beta(+) T cells. We showed that AhR is required for IL-22 production by Th17, but not by the three other cell types, in the imiquimod-treated ears. Moreover, AhR has a role in the recruitment of gamma delta T cells, ILC3, and CD4(-)CD8(-)TCR beta(+) T cells into the inflamed skin or in their local proliferation. Taken together, AhR has a direct role in IL-22 production by Th17 cells in the mouse ear skin, but not by gamma delta T cells, CD4(-)CD8(-)TCR beta(+) T cells and ILCs.