EXPRESSION OF PHEROMONE BIOSYNTHESIS ACTIVATING NEUROPEPTIDE AND ITS RECEPTOR (PBANR) mRNA IN ADULT FEMALE Spodoptera exigua (LEPIDOPTERA: NOCTUIDAE)

摘要

The full-length cDNA of pheromone biosynthesis activating neuropeptide receptor (PBANR) was cloned from the beet armyworm, Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae); it included an open reading frame of 1,053 bp encoding 350 amino acids. ThePBANR of S. exigua (SePBANR) was structurally characteristic of G protein–coupled receptor and its amino acid sequence shared 98% identity with the PBANR of Spodoptera littoralis. Both pheromone biosynthesis activating neuropeptide (PBAN) and PBANR mRNA abundance were measured in the brain-subesophageal ganglion complex, pheromone gland, ventral nerve cord, and ovary of S. exigua female moths by real-time RT-PCR. The abundance of PBAN mRNA in brain-subesophageal ganglion complex and PBANR mRNA in pheromone gland was significantly greater compared to other tissues, suggesting that the ligand-receptor relationship of PBAN and PBANR exists quantitatively in S. exigua.Both PBAN and PBANR expression displayed a remarkable diurnal rhythm, for they were low and stable during the photophase