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Anti-wrinkle and anti-whitening effects of juca (Libidibia ferrea Mart.) extracts

机译:Juca(Libidibia ferrea Mart。)提取物的抗皱和美白功效

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Skin aging is a natural process of the human body that may be accelerated due to extrinsic causes. Libidibia ferrea, popularly known as juca, is a small tree, which possesses an abundant phenolic composition with potential antioxidant and enzymatic inhibition activities. Thus, this work aimed to investigate the anti-wrinkle and anti-whitening potentials of juca trunk bark (LFB) and pod (LFP) extracts. A comprehensive analysis of LFB and LFP phenolic composition was accomplished by means of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Effects on skin degradation were assessed by inhibitory enzymatic activity against elastase, hyaluronidase and collagenase through colorimetric assays. Cellular viability in B16F10 and primary fibroblasts were determined by Trypan Blue exclusion assay. Anti-melanogenic effects on B16F10 cells were evaluated using cellular tyrosinase, melanin content, western blot, and RT-qPCR analyses. Inhibition of matrix metalloproteinase-2 and metalloproteinase-9 (MMP-2 and MMP-9) was determined by gelatin zymography and western blot methodologies. LC-MS/MS analyses of LFB and LFP extracts allowed the characterization of 18 compounds, among them, flavonoids, phenolic acids, and secoridoids. Additionally the pod and trunk bark compositions were compared. Hyaluronidase inhibitory activity for both extracts, LFB (IC50 = 8.5 +/- 0.8 A mu g/mL) and LFP (IC50 = 16 +/- 0.5 A mu g/mL), was stronger than standard rutin (IC50 = 27.6 +/- 0.06). Pro-MMP-2 was significantly inhibited by both extracts. LFB and LFP decreased the melanin content in B16F10 due to tyrosinase inhibitory activity. L. ferrea extracts has high potential as a cosmetic ingredient due to its anti-wrinkle and depigmentant effects.
机译:皮肤衰老是人体的自然过程,由于外在原因可能会加速衰老。 Libidibia ferrea,俗称juca,是一棵小树,它具有丰富的酚类成分,具有潜在的抗氧化剂和酶促抑制活性。因此,这项工作旨在研究朱卡树干皮(LFB)和豆荚(LFP)提取物的抗皱和抗美白潜力。通过液相色谱-串联质谱(LC-MS / MS)对LFB和LFP酚类成分进行了全面分析。通过比色测定法对弹性蛋白酶,透明质酸酶和胶原酶的抑制酶活性来评估对皮肤降解的影响。通过台盼蓝排除法测定B16F10和原代成纤维细胞的细胞活力。使用细胞酪氨酸酶,黑色素含量,蛋白质印迹和RT-qPCR分析评估了对B16F10细胞的抗黑色素生成作用。通过明胶酶谱法和蛋白质印迹法确定对基质金属蛋白酶2和金属蛋白酶9(MMP-2和MMP-9)的抑制作用。 LC-MS / MS对LFB和LFP提取物的分析可以表征18种化合物,其中包括黄酮类化合物,酚酸和类硒油化合物。此外,比较了豆荚和树干的树皮成分。 LFB(IC50 = 8.5 +/- 0.8 Aμg / mL)和LFP(IC50 = 16 +/- 0.5 Aμg/ mL)两种提取物的透明质酸酶抑制活性均强于标准芦丁(IC50 = 27.6 + / -0.06)。两种提取物均显着抑制了Pro-MMP-2。 LFB和LFP由于酪氨酸酶抑制活性而降低了B16F10中黑色素的含量。由于其抗皱和脱色作用,L。ferrea提取物具有作为化妆品成分的高潜力。

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