Assessment of serum IL-1, IL-2 and IFN-gamma levels in untreated pulmonary tuberculosis patients: role in pathogenesis.

摘要

BACKGROUND AND AIMS: Tuberculosis (Tb) infection is controlled by cell-mediated immunity through mediation of IL-1, IL-2 and IFN-gamma. In this study IL-1, IL-2 and IFN-gamma were determined in serum samples of untreated pulmonary Tb and control group including apparently healthy individuals or contacts and normal healthy blood donors with an objective of understanding defect(s), if any, in synthesis of any of these cytokines that may lead to a diseased state of Tb. METHODS: IL-1, IL-2 and IFN-gamma were measured in serum samples of untreated Tb patients (n=33), contacts (n=19) and healthy individuals (n=20) by commercially available monoclonal antibody-based ELISA. RESULTS: Statistically significant differences in IL-1 and IFN-gamma concentrations between groups of pulmonary Tb and controls were observed, whereas no significant difference in IL-2 was seen. CONCLUSIONS: In the present study, increased levels of cytokines in patients with pulmonary Tb are indicative of Th1 response. An increased level of cytokine (IFN-gamma) in patients with untreated pulmonary Tb appears to be functionally defective.