Isolated mitochondria infusion mitigates ischemia-reperfusion injury of the liver in rats: reply.

摘要

In our previous study, we showed that infusion of freshly isolated mitochondria via spleen significantly mitigated ischemia-reperfusion injury of the liver. We used JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide) staining and flow cytometry analysis to examine the membrane potential of the isolated mitochondria (DELTAPSIm) before the infusion. Besides, we used MitoTracker Orange CMTMRos (5 MUmoL/L; Invitrogen, Carlsbad, Calif) to stain the isolated mitochondria and track their biodistribution in the liver. After 240 min of reperfusion, we observed the MitoTracker Orange-labeled mitochondria distributed among the cryo-sectioned liver parenchyma. We proposed that the infused mitochondria still maintained their membrane potential after infusion.